Bioanalytical Techniques specifically relates to determine the concentration of drug in the biological matrices. The quantitative determination of drug and metabolic in biological material to establish in pharma kinetic and toxicokinetic studies. It also deals with the human clinical pharmacology, bioavailability, bioequivalence and non-human clinical pharmacology. Separation process involved between bio-molecules like proteins, amino acids, sugars, cells, tissue. Bioseparation usually involves the separation of the following physical forms.